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FAQs: NutriScan Diagnostic Test for Food Sensitivity & Intolerance

Q. What is the NutriScan test?

A.  This test measures antibodies to certain foods in dog saliva. High antibody
levels indicate that the dog has a food sensitivity and intolerance to that food
or foods.  It is not a DNA cheek swab test.

Q. Does the test work in species other than dogs ?

A.  No, this is a dog-specific test. NutriScan hopes to extend testing to cats and
horses in future.

Q. Does this test apply to healthy dogs as well as those with known or suspected food reactivitiy?

A.  Yes, because saliva testing can reveal the latent or pre-clinical form of food
sensitivity, it can be used to predict a developing or latent food reaction.

Q. How does this test differ from other food “allergy” tests on serum or feces ?

A. Food allergy tests measure antibodies to IgG and IgE in serum or feces. These are typically more acute allergic reactions to foods, whereas NutriScan measures IgA and IgM antibodies on the bowel’s mucosal surface, and thus more directly correlates to symptoms of bowel (GI tract) disease.

Q. How does this test differ from other food “allergy” skin testing ?

A. Skin testing used to be considered the “gold standard” of allergy testing. Aside from being unsightly and requiring that a large patch of skin be shaved, these tests are costly and do not always identify the true source of allergic reactions.

Q. Why is salivary food sensitivity and intolerance testing more predictive than other food allergy testing ?

A. Saliva testing can reveal the latent or pre-clinical form of food sensitivity,as antibodies to food ingredients appear in saliva before the clinical or bowel biopsydiagnosis of inflammatory bowel disease or “leaky gut syndrome” is made.

Q. When do these food sensitivities typically appear once an offending food is eaten?

A. Food sensitivities are usually seen from as early as 2 hrs and up to 72 hrs after eating, so it can be difficult to connect symptoms with a food or foods eaten several days previously. There is a very high correlation between delayed food sensitivity and the amount and frequency of the food consumed.

Q. What foods are tested ?

A. Six primary food antigens were tested originally: beef, corn, hen’s eggs, cow’s milk,soy and wheat. This test will be expanded in early 2012 to include two different panels each having 10 food antigens.   This will be the first panel of 10 foods = beef, corn, wheat, soy, cow’s milk, lamb, venison, chicken, turkey, and white fish.   The second panel = hen’s egg, barley, millet, oatmeal, salmon, rabbit, rice, quinoa, potato, and peanut (peanut butter). You’ll be able to order one or both panels simultaneously or sequentially.

Q. Should my dog be fasted or not eat before collecting the saliva?

A.  Fasting in not necessary, but the dog should not eat anything for at least 3 hrs
before saliva collection.

Q. How much saliva is needed ?

A. The test requires 2 mL of saliva so it can be run in duplicate. It is important to collect enough saliva.

Q. Must the dental cotton rope be wet with saliva?

A.   Yes, about half of the length of the rope should be wet with saliva.

Q. What can I do if my dog won’t salivate ?

A.  You can place a piece of tempting food in front of the dog’s nose to
stimulate salivation, but don’t let him actually eat the treat.

Q. Does it matter if there are some food particles on the rope ?

A. Please try to minimize this contamination .

Q. Does the sample need to be frozen or specially packed for shipping to Hemopet ?

A. No, room temperature is fine.

Q. How long can the sample be stored before shipping or being assayed ?

A. Salivary antigens are stable for up to 30 days after collection, and we can 
re-hydrate the rope with a small measured amount of saline, if needed.

Q. What does the test measure ?

A. Two types of antibodies (anti-IgA and anti-IgM) against different purified
food antigen extracts are measured

Q. What should I do if foods test reactive?

Avoid foods, treats, supplements, grooming supplies and drug vehicles (substances  to administer drugs)  that contain these ingredients.

Q. How is NutriScan reported?

A.  Results are quantified in Units per ml of saliva

Q. What do the Units of food reactivity represent ?

A.  The antibody levels present in custom-made immunoassay plates are measured
by optical density readings and these are converted to Units per ml of reaction.

Q. What is a negative reaction ?

A. Negative reactions measure less than 10 Units per ml of saliva.

Q. What are weak and moderately high food reactions ?

A. Those between 10.00-12.99 Units per ml are considered weak reactions; those from 13.00 to 14.99 are moderately high reactions.

Q. What do the weak reactions mean ?

A. The clinical significance of the weak reactions is unclear, but in dogs with established clinical signs of food sensitivity, it would be prudent to avoid foods reacting weakly as well.

Q. How often should I repeat the test ?

A.  We suggest repeat testing every 6-12 months in healthy dogs, and every
4-6 months in food reactive dogs.

Q. What are strong food reactions?

A.  Those at or above 15.00 Units per ml are strong reactions.

Q. If my dog tests reactive to beef, will he react to bison or buffalo ?

A.  Presumably not, but there are no data on this point.

Q. If my dog tests reactive to cow’s milk, will he react to goat or sheep milk?

A.  Presumably not, but there are no data on this point.

Q. If my dog tests reactive to cow’s milk, will he likely react to cheese made from milk ?

A.  No, he shouldn’t as most, but not all, cheese is made from the whey fraction of milk.

Q. If my dog tests reactive to hen’s eggs, will he likely react to chicken ?

A.   Presumably, he also will react to chicken

Q. What did the initial clinical trials with this test panel reveal ?

A.  Clinical trials included 566 dogs; each was tested with the 6 primary food antigens for anti-IgA and anti-IgM in saliva and anti-IgG in serum.

  • 62% (352 of the 566) of the dogs tested showed weak, moderate or strong food reactivity to at least one food allergen.
  • 71% of the dogs tested showed weak, moderate or strong food reactivity to beef.
  • 71% of the dogs tested showed weak, moderate or strong food reactivity to wheat.
  • 70% of the dogs tested showed moderate or strong food reactivity to cow’s milk.
  • 57% of the dogs tested showed weak, moderate or strong food reactivity to corn.
  • Fewer dogs showed food reactivity to soy (25%) and very few to egg (3%).
  • Only one dog of 121 control dogs showed a mild anti-IgG reaction in serum, and only to wheat.

Q. Why did we select only 20 food allergens for salivary food sensitivity and intolerance testing?

We have  selected the so-called 6 primary food antigens, including the glutens present in the most commonly fed pet foods and treats and an addtional 14 antigens which can be found in some pet foods.  If there is a food sensitivity or intolerance, it is very likely going to be to one of these antigens.

Q. Are more needed ?

No and Yes.  Other testing on serum includes about 20 foods, but our saliva testing provides more predictable clinical  outcomes.   In the  economic downturn we now face, we need to be more discriminating about what testing is really needed.  So this saliva test is a reliable and cost-effective screening test which can give you  useful  information. We do not anticipate that further panels will be needed.

Q. What food antigens are in the two panels?

There are  2 panels: each with 10 antigens:

Panel 1  = beef, corn, wheat, soy, cow’s milk, lamb, venison, chicken, turkey, and white fish.

Panel 2 = hen’s egg, barley, millet, oatmeal, salmon, rabbit, rice, quinoa, potato, and peanut (peanut butter).

You can order one or both panels simultaneously or sequentially.

Q. What testing should I do ?

The choice is yours: We can do:

Panel 1 only

Panel 2 only

Panel 1, and if there are no or few reactivities shown there, follow with Panel 2

Panels 1 and 2 at the same  time

But above all, you should know:  we test for the antibodies critical to determining food sensitivity and intolerance, namely, IgA and IgM—in saliva, not in serum. No one else does this.    Our focused salivary testing using the clinically relevant  antibodies provides scientifically proven, novel veterinary diagnostics.


NutriScan  technology is patented in the US. Other US and international patents are pending. Hemopet, Hemoilfe and NutriScan are Registered Trademarks.